nPLEX SARS-CoV-2 with dUTP is a multiplex real-time PCR test for the detection of coronavirus in respiratory samples, such as nasopharyngeal, oropharyngeal and nasal swabs. This kit contains two PCR mixes. The first one has primers and probes for the simultaneous detection of both the N1 region of the nucleocapsid gene N (detection of SARS-CoV-2) and the human RNAse P gene RP, which serves as an internal control. The second PCR mix is a confirmatory assay, to be used in cases in which the positive results are weak, and it contains primers and probes for the detection of the N2 region of the nucleocapsid gene N of SARS-CoV-2. This kit has been extensively validated for SARS-CoV-2 RNA extraction from clinical samples (over 50.000 clinical samples). In addition, the PCR mixes contain dUTP instead of dTTP, to prevent amplicon-based contamination in your workflow.